Protocols

Below is a lab notebook video that discusses best practices for retaining research records. Additionally, there are links to some of our most common lab protocols. (We do not endorse any of the listed suppliers.)

Nucleic Acid Nanoparticle (NANP) Assembly and Characterization

PCR

RNA Purification and Recovery

DNA Templates Purification

NANP Assembly

In Vitro Transcription

NANP Assembly Verification

Mammalian Cell Work and Assays used to investigate NANPs

Cell Maintenance

HEK-Blue (TLR3,7,8, 9) Assay

HEK-Lucia (RIG-I) Assay

Flow Cytometry
Set-Up

cGAS Inhibition Assay

FBS Stability

Assay

TGGE

MTS Assay

Bacterial Cell Work and Assays used to investigate NANPs

Starting a Bacterial Culture

Excitation Emission Matrix (EEM)